Abstract
A technique for creating arrays of parallel, stretched single molecules of deoxyribose nucleic acid (DNA) on an arbitrary substrate for high-resolution scanning-probe imaging is discussed. The technique consists of lithographically patterning polystyrene lines on a substrate which then provide attachment sites for the ends of individual DNA molecules. Molecular combing is performed to stretch DNA from one polystyrene line to the other. Scanning-tunneling and atomic-force microscope images of single molecules of bacteriophage-lambda DNA are shown to demonstrate the advantages of this technique. Several applications, from high-resolution genomics to molecular electronics, are discussed.
Original language | English |
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Journal | Applied Physics Letters |
Volume | 78 |
Issue number | 16 |
Pages (from-to) | 2396-2398 |
Number of pages | 3 |
ISSN | 0003-6951 |
DOIs | |
Publication status | Published - 16 Apr 2001 |