Growth and single cell kinetics of the loricate choanoflagellate Diaphanoeca grandis

Niels Thomas Eriksen, Jakob Tophøj, Rasmus Dam Wollenberg, Peter Funch, Per Andersen

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

Resumé

Choanoflagellates are common members of planktonic communities. Some have complex life histories that involve transitions between multiple cell stages. We have grown the loricate choanoflagellate Diaphanoeca grandis on the bacterium Pantoea sp. and integrated kinetic observations at the culture level and at the single cell level. The life history of D. grandis includes a cell division cycle with a number of recognisable cell stages. Mature, loricate D. grandis were immobile and settled on the bottom substratum. Daughter cells were ejected from the lorica 30 min. after cell division, became motile and glided on the bottom substratum until they assembled a lorica. Single cell kinetics could explain overall growth kinetics in D. grandis cultures. The specific growth rate was 0.72 day −1 during exponential growth while mature D. grandis produced daughter cells at a rate of 0.9 day −1. Daughter cells took about 1.2 h to mature. D. grandis was able to abandon and replace its lorica, an event that delayed daughter cell formation by more than 2 days. The frequency of daughter cell formation varied considerably among individuals and single cell kinetics demonstrated an extensive degree of heterogeneity in D. grandis cultures, also when growth appeared to be balanced.

OriginalsprogEngelsk
Artikelnummer14543
TidsskriftScientific Reports
Vol/bind9
Sider (fra-til)1
Antal sider12
ISSN2045-2322
DOI
StatusUdgivet - 2019

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Diaphanoeca
kinetics
cells
cell division
life history
Pantoea
specific growth rate

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title = "Growth and single cell kinetics of the loricate choanoflagellate Diaphanoeca grandis",
abstract = "Choanoflagellates are common members of planktonic communities. Some have complex life histories that involve transitions between multiple cell stages. We have grown the loricate choanoflagellate Diaphanoeca grandis on the bacterium Pantoea sp. and integrated kinetic observations at the culture level and at the single cell level. The life history of D. grandis includes a cell division cycle with a number of recognisable cell stages. Mature, loricate D. grandis were immobile and settled on the bottom substratum. Daughter cells were ejected from the lorica 30 min. after cell division, became motile and glided on the bottom substratum until they assembled a lorica. Single cell kinetics could explain overall growth kinetics in D. grandis cultures. The specific growth rate was 0.72 day −1 during exponential growth while mature D. grandis produced daughter cells at a rate of 0.9 day −1. Daughter cells took about 1.2 h to mature. D. grandis was able to abandon and replace its lorica, an event that delayed daughter cell formation by more than 2 days. The frequency of daughter cell formation varied considerably among individuals and single cell kinetics demonstrated an extensive degree of heterogeneity in D. grandis cultures, also when growth appeared to be balanced.",
author = "Eriksen, {Niels Thomas} and Jakob Toph{\o}j and Wollenberg, {Rasmus Dam} and Peter Funch and Per Andersen",
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Growth and single cell kinetics of the loricate choanoflagellate Diaphanoeca grandis. / Eriksen, Niels Thomas; Tophøj, Jakob; Wollenberg, Rasmus Dam; Funch, Peter; Andersen, Per.

I: Scientific Reports, Bind 9, 14543, 2019, s. 1.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

TY - JOUR

T1 - Growth and single cell kinetics of the loricate choanoflagellate Diaphanoeca grandis

AU - Eriksen, Niels Thomas

AU - Tophøj, Jakob

AU - Wollenberg, Rasmus Dam

AU - Funch, Peter

AU - Andersen, Per

PY - 2019

Y1 - 2019

N2 - Choanoflagellates are common members of planktonic communities. Some have complex life histories that involve transitions between multiple cell stages. We have grown the loricate choanoflagellate Diaphanoeca grandis on the bacterium Pantoea sp. and integrated kinetic observations at the culture level and at the single cell level. The life history of D. grandis includes a cell division cycle with a number of recognisable cell stages. Mature, loricate D. grandis were immobile and settled on the bottom substratum. Daughter cells were ejected from the lorica 30 min. after cell division, became motile and glided on the bottom substratum until they assembled a lorica. Single cell kinetics could explain overall growth kinetics in D. grandis cultures. The specific growth rate was 0.72 day −1 during exponential growth while mature D. grandis produced daughter cells at a rate of 0.9 day −1. Daughter cells took about 1.2 h to mature. D. grandis was able to abandon and replace its lorica, an event that delayed daughter cell formation by more than 2 days. The frequency of daughter cell formation varied considerably among individuals and single cell kinetics demonstrated an extensive degree of heterogeneity in D. grandis cultures, also when growth appeared to be balanced.

AB - Choanoflagellates are common members of planktonic communities. Some have complex life histories that involve transitions between multiple cell stages. We have grown the loricate choanoflagellate Diaphanoeca grandis on the bacterium Pantoea sp. and integrated kinetic observations at the culture level and at the single cell level. The life history of D. grandis includes a cell division cycle with a number of recognisable cell stages. Mature, loricate D. grandis were immobile and settled on the bottom substratum. Daughter cells were ejected from the lorica 30 min. after cell division, became motile and glided on the bottom substratum until they assembled a lorica. Single cell kinetics could explain overall growth kinetics in D. grandis cultures. The specific growth rate was 0.72 day −1 during exponential growth while mature D. grandis produced daughter cells at a rate of 0.9 day −1. Daughter cells took about 1.2 h to mature. D. grandis was able to abandon and replace its lorica, an event that delayed daughter cell formation by more than 2 days. The frequency of daughter cell formation varied considerably among individuals and single cell kinetics demonstrated an extensive degree of heterogeneity in D. grandis cultures, also when growth appeared to be balanced.

U2 - 10.1038/s41598-019-50998-0

DO - 10.1038/s41598-019-50998-0

M3 - Journal article

VL - 9

SP - 1

JO - Scientific Reports

JF - Scientific Reports

SN - 2045-2322

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ER -