TY - JOUR
T1 - Vertical distribution of bacterioplankton in Lake Averno in relation to water chemistry
AU - Paganin, Patrizia
AU - Chiarini, Luigi
AU - Bevivino, Annamaria
AU - Dalmastri, Claudia
AU - Farcomeni, Alessio
AU - Izzo, Giulio
AU - Signorini, Antonella
AU - Varrone, Cristiano
AU - Tabacchioni, Silvia
PY - 2013/4
Y1 - 2013/4
N2 - The effects of environmental factors on bacterioplankton distribution along the water column of Lake Averno (Naples, Italy) have been investigated by means of denaturing gradient gel electrophoresis (DGGE) of polymerase chain reaction (PCR)-amplified 16S rRNA gene fragments, and multivariate analysis applied to molecular data and physico-chemical parameters. Bacterial richness, estimated from DGGE profiles, remains constant throughout the water column, whereas archaeal richness increases with depth. Moreover, archaeal richness was significantly correlated to most of the measured abiotic variables, whereas bacterial richness did not. Analysis of sequences from DGGE bands revealed that the dominant bacterial populations belong to Alphaproteobacteria, Betaproteobacteria, Deltaproteobacteria, Bacteroidetes and Firmicutes, whereas sequences of the archaeal DGGE bands are affiliated to Methanomicrobiales and Methanosarcinales orders. Moreover, bacterial sequences affiliated to Proteobacteria and Bacteroidetes do not group closely to typical freshwater lineages/clades/tribes within these phyla. Bacterioplankton distribution along the water column was apparently correlated with the vertical gradient of physico-chemical parameters as a statistically significant relationship between most of them with grouping of specific taxonomic units was observed.
AB - The effects of environmental factors on bacterioplankton distribution along the water column of Lake Averno (Naples, Italy) have been investigated by means of denaturing gradient gel electrophoresis (DGGE) of polymerase chain reaction (PCR)-amplified 16S rRNA gene fragments, and multivariate analysis applied to molecular data and physico-chemical parameters. Bacterial richness, estimated from DGGE profiles, remains constant throughout the water column, whereas archaeal richness increases with depth. Moreover, archaeal richness was significantly correlated to most of the measured abiotic variables, whereas bacterial richness did not. Analysis of sequences from DGGE bands revealed that the dominant bacterial populations belong to Alphaproteobacteria, Betaproteobacteria, Deltaproteobacteria, Bacteroidetes and Firmicutes, whereas sequences of the archaeal DGGE bands are affiliated to Methanomicrobiales and Methanosarcinales orders. Moreover, bacterial sequences affiliated to Proteobacteria and Bacteroidetes do not group closely to typical freshwater lineages/clades/tribes within these phyla. Bacterioplankton distribution along the water column was apparently correlated with the vertical gradient of physico-chemical parameters as a statistically significant relationship between most of them with grouping of specific taxonomic units was observed.
KW - Archaea
KW - Bacteria
KW - Denaturing gradient gel electrophoresis
KW - Environmental parameters
KW - Lake water column
KW - Nonlinear multidimensional scaling
UR - http://www.scopus.com/inward/record.url?scp=84874669872&partnerID=8YFLogxK
U2 - 10.1111/1574-6941.12048
DO - 10.1111/1574-6941.12048
M3 - Journal article
AN - SCOPUS:84874669872
SN - 0168-6496
VL - 84
SP - 176
EP - 188
JO - FEMS Microbiology Ecology
JF - FEMS Microbiology Ecology
IS - 1
ER -