Who needs neighbors? PKS8 is a Stand-Alone Gene in Fusarium graminearum responsible for production of Gibepyrones and Prolipyrone B

Klaus Ringsborg Westphal; Asmus Toftkær Muurmann; Iben Engell Paulsen; Kim Tanja Hejselbak Nørgaard; Marie Lund Overgaard; Sebastian Mølvang Dall; Trine Aalborg; Reinhard Wimmer; Jens Laurids Sørensen; Teis Esben Sondergaard

doi:10.3390/molecules23092232

Who needs neighbors? PKS8 is a Stand-Alone Gene in Fusarium graminearum responsible for production of Gibepyrones and Prolipyrone B

Klaus Ringsborg Westphal, Asmus Toftkær Muurmann, Iben Engell Paulsen, Kim Tanja Hejselbak Nørgaard, Marie Lund Overgaard, Sebastian Mølvang Dall, Trine Aalborg, Reinhard Wimmer, Jens Laurids Sørensen, Teis Esben Sondergaard^*

^*Corresponding author for this work

Research output: Contribution to journal › Journal article › Research › peer-review

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Abstract

Genome sequencing of the genus Fusarium has revealed a great capacity for discovery of new natural products of potential economical and therapeutic importance. Several of these are unknown. In this study, we investigated the product of the PKS8 gene in Fusarium graminearum, which was recently linked to gibepyrones in F. fujikuroi. Genomic analyses showed that PKS8 constitutes a stand-alone gene in F. graminearum and related species. Overexpression of PKS8 resulted in production of gibepyrones A, B, D, G and prolipyrone B, which could not be detected in the wild type strain. Our results suggest that PKS8 produces the entry compound gibepyrone A, which is subsequently oxidized by one or several non-clustering cytochrome P450 monooxygenases ending with prolipyrone B.

Original language	English
Article number	2232
Journal	Molecules
Volume	23
Issue number	9
ISSN	1420-3049
DOIs	https://doi.org/10.3390/molecules23092232
Publication status	Published - 2 Sept 2018

Keywords

Fusarium
Gibepyrones
Polyketide synthases
Prolipyrone
Secondary metabolites
Multigene Family
Oxidation-Reduction
Polyketide Synthases/genetics
Pyrones/chemistry
Proton Magnetic Resonance Spectroscopy
Fusarium/enzymology
Biosynthetic Pathways/genetics
Genes, Fungal

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10.3390/molecules23092232Licence: CC BY 4.0

Open Access articleFinal published version, 3.15 MBLicence: CC BY 4.0

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Westphal, K. R., Muurmann, A. T., Paulsen, I. E., Nørgaard, K. T. H., Overgaard, M. L., Dall, S. M., Aalborg, T., Wimmer, R., Sørensen, J. L., & Sondergaard, T. E. (2018). Who needs neighbors? PKS8 is a Stand-Alone Gene in Fusarium graminearum responsible for production of Gibepyrones and Prolipyrone B. Molecules, 23(9), Article 2232. https://doi.org/10.3390/molecules23092232

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abstract = "Genome sequencing of the genus Fusarium has revealed a great capacity for discovery of new natural products of potential economical and therapeutic importance. Several of these are unknown. In this study, we investigated the product of the PKS8 gene in Fusarium graminearum, which was recently linked to gibepyrones in F. fujikuroi. Genomic analyses showed that PKS8 constitutes a stand-alone gene in F. graminearum and related species. Overexpression of PKS8 resulted in production of gibepyrones A, B, D, G and prolipyrone B, which could not be detected in the wild type strain. Our results suggest that PKS8 produces the entry compound gibepyrone A, which is subsequently oxidized by one or several non-clustering cytochrome P450 monooxygenases ending with prolipyrone B.",

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AU - Nørgaard, Kim Tanja Hejselbak

AU - Overgaard, Marie Lund

AU - Dall, Sebastian Mølvang

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AU - Sørensen, Jens Laurids

AU - Sondergaard, Teis Esben

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AB - Genome sequencing of the genus Fusarium has revealed a great capacity for discovery of new natural products of potential economical and therapeutic importance. Several of these are unknown. In this study, we investigated the product of the PKS8 gene in Fusarium graminearum, which was recently linked to gibepyrones in F. fujikuroi. Genomic analyses showed that PKS8 constitutes a stand-alone gene in F. graminearum and related species. Overexpression of PKS8 resulted in production of gibepyrones A, B, D, G and prolipyrone B, which could not be detected in the wild type strain. Our results suggest that PKS8 produces the entry compound gibepyrone A, which is subsequently oxidized by one or several non-clustering cytochrome P450 monooxygenases ending with prolipyrone B.

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Who needs neighbors? PKS8 is a Stand-Alone Gene in Fusarium graminearum responsible for production of Gibepyrones and Prolipyrone B

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