TY - JOUR
T1 - Herpes simplex virus infection of cultured human term trophoblast
AU - Nørskov‐Lauritsen, N.
AU - Aboagye‐Mathisen, G.
AU - Juhl, C. B.
AU - Petersen, P. M.
AU - Zachar, V.
AU - Ebbesen, P.
PY - 1992/3
Y1 - 1992/3
N2 - Mononuclear trophoblast cells were isolated from term placentas of uncomplicated pregnancies, purified to homogeneity by negative immunomagnetic separation using monoclonal antibodies to the major histocompatibility complex, and challenged with herpes simplex virus (HSV). The cultures were highly susceptible to virusinduced cytopathic effect as evidenced by cytopathic alteration and inhibition of human chorionic gonadotropin (hCG) secretion. Both HSV I and II underwent a full replicative cycle in the trophoblast, although the production of progeny virus was 10‐100 times less than that obtained with placental fibroblasts or choriocarcinoma cells. The permissiveness was independent of in vitro syncytial differentiation of the trophoblast. The results suggest that the trophoblast layer may be involved in intrauterine HSV infection.
AB - Mononuclear trophoblast cells were isolated from term placentas of uncomplicated pregnancies, purified to homogeneity by negative immunomagnetic separation using monoclonal antibodies to the major histocompatibility complex, and challenged with herpes simplex virus (HSV). The cultures were highly susceptible to virusinduced cytopathic effect as evidenced by cytopathic alteration and inhibition of human chorionic gonadotropin (hCG) secretion. Both HSV I and II underwent a full replicative cycle in the trophoblast, although the production of progeny virus was 10‐100 times less than that obtained with placental fibroblasts or choriocarcinoma cells. The permissiveness was independent of in vitro syncytial differentiation of the trophoblast. The results suggest that the trophoblast layer may be involved in intrauterine HSV infection.
KW - fetal infection
KW - immunosorting
KW - transplacental
KW - vertical
KW - virus‐transmission
UR - http://www.scopus.com/inward/record.url?scp=0026503443&partnerID=8YFLogxK
U2 - 10.1002/jmv.1890360303
DO - 10.1002/jmv.1890360303
M3 - Journal article
C2 - 1314282
AN - SCOPUS:0026503443
SN - 0146-6615
VL - 36
SP - 162
EP - 166
JO - Journal of Medical Virology
JF - Journal of Medical Virology
IS - 3
ER -